Table 3.

Catalase abrogates the inhibitory activity of 2-OH^a

Additive (amt or concn)				Growthb
Catalase (U)	2-OH (μM)	BSA (μg)	H2O2 (mM)
0	0	370	0	+
10,000	0	0	0	+
0	60	0	0	−
5,000	60	0	0	+
0	60	370	0	−
0	0	0	0.5	+
0	0	0	1	+
0	0	0	1.5	−
0	0	0	2	−
5,000	0	0	2	+

^aAF293 spores (1 × 10³ total CFU) were added to wells with the indicated additives. Addition of 60 μM 2-OH resulted in growth inhibition of spores similar to that caused by addition of 1.5 mM H₂O₂. Bovine catalase at a concentration of 5,000 units/well was able to eliminate the growth inhibition by 2-OH and H₂O₂.

^bThe symbols + and − denote growth and no growth, respectively. Growth was assessed by microscopic visualization of wells 48 h posttreatment.