Table 3.
Additive (amt or concn) |
Growthb | |||
---|---|---|---|---|
Catalase (U) | 2-OH (μM) | BSA (μg) | H2O2 (mM) | |
0 | 0 | 370 | 0 | + |
10,000 | 0 | 0 | 0 | + |
0 | 60 | 0 | 0 | − |
5,000 | 60 | 0 | 0 | + |
0 | 60 | 370 | 0 | − |
0 | 0 | 0 | 0.5 | + |
0 | 0 | 0 | 1 | + |
0 | 0 | 0 | 1.5 | − |
0 | 0 | 0 | 2 | − |
5,000 | 0 | 0 | 2 | + |
AF293 spores (1 × 103 total CFU) were added to wells with the indicated additives. Addition of 60 μM 2-OH resulted in growth inhibition of spores similar to that caused by addition of 1.5 mM H2O2. Bovine catalase at a concentration of 5,000 units/well was able to eliminate the growth inhibition by 2-OH and H2O2.
The symbols + and − denote growth and no growth, respectively. Growth was assessed by microscopic visualization of wells 48 h posttreatment.