Table 1.
16S rRNA gene similarities of SSCP bands identified exclusively in the sea surface microlayer
| Taxon and OTUa | Station(s)b | Fractionc | Relative band intensity (%) | No. of sequences | Closest phylogenetic relatived |
Phylogenetic affiliatione | GenBank accession no. (this study) | |||
|---|---|---|---|---|---|---|---|---|---|---|
| Name | Accession no. | 16S rRNA sequence similarity (%) | Habitat | |||||||
| Alphaproteobacteria | ||||||||||
| BaSML1 | 6 | N | 3.9 | 1 | Uncultured bacterium clone 1C227571 | EU799901 | 98 | Marine harbor | Rhodospirillales | HM193051 |
| BaSML2 | 9 | N | 2.2 | 1 | Uncultured alphaproteobacterium clone CB51E03 | EF471665 | 100 | Coastal water | Rhodobacteraceae | HM193053 |
| BaSML3 | 9 | P | 3.6 | 1 | Uncultured alphaproteobacterium clone CB51E03 | EF471665 | 98 | Coastal water | Rhodobacteraceae | HM193073 |
| BaSML4 | 11 | P | 0.9 | 1 | Uncultured alphaproteobacterium clone 131718 | AY922222 | 99 | Marine microbial mat | Rhodobacteraceae | HM193064 |
| BaSML5 | 4 | N | 3.3 | 2 | Alphaproteobacterium MPCa6SU_Cu07 isolate | EF414048 | 100 | Sponge associated | Sphingomonadaceae | HM193058 |
| Betaproteobacteria | ||||||||||
| BaSML6 | 17 | N | 3.5 | 1 | Uncultured bacterium clone TBb-04 | DQ791365 | 98 | Geothermally heated soil | Burkholderiales | HM193061 |
| BaSML7 | 15 | P | 0.5 | 1 | Uncultured Pelomonas sp. clone B6 | EF125953 | 99 | Biofilm reactor | Burkholderiales | HM193071 |
| BaSML8 | 7, 8 | N | 3.5 | 2 | Uncultured betaproteobacterium isolate, SSCP band 77-7-15 | GU088518 | 100 | Tropical lagoon water | Burkholderiales | HM193052 |
| Gammaproteobacteria | ||||||||||
| BaSML9 | 4 | N | 3.6 | 2 | Rheinheimera sp. JSM 083085 | FJ527418 | 100 | Forest soil | Chromatiaceae | HM193056 |
| BaSML10 | 9 | N/P | 2.8 | 2 | Rheinheimera sp. 3006 | AM110966 | 99 | Deep-sea sediment | Chromatiaceae | HM193072 |
| BaSML11 | 4 | P | 3.4 | 1 | Rheinheimera sp. enrichment culture clone AM15 | FJ516460 | 99 | Enrichment culture | Chromatiaceae | HM193081 |
| BaSML12 | 19 | P | 7.8 | 1 | Uncultured bacterium clone CCSD_DF2700_B7 | DQ128248 | 100 | Drilling fluid | Chromatiaceae | HM193082 |
| BaSML13 | 1 | P | 7.7 | 1 | Buchnera aphidicola strain 5A | CP001161 | 99 | Endosymbiont (insect) | Enterobacteriaceae | HM193076 |
| BaSML14 | 2 | P | 6.3 | 3 | Buchnera aphidicola | M63248 | 99 | Endosymbiont (insect) | Enterobacteriaceae | HM193078 |
| BaSML15 | 5 | N | 0.8 | 1 | Uncultured bacterium clone C7p3_ML_171 | FJ353210 | 98 | Lake water | Halothiobacillaceae | HM193050 |
| BaSML16 | 1 | P | 1.8 | 1 | Eubacterium from Cecidotrioza sozanica | AF286124 | 97 | Associated with insects | Coxiellaceae | HM193075 |
| BaSML17 | 1, 17 | P | 3.2 | 2 | Uncultured gammaproteobacterium clone SR-O-03-32 | AM905315 | 98 | Beach sediment | Moraxellaceae | HM193077 |
| BaSML18 | 17 | N | 0.3 | 1 | Shewanella sp. enrichment culture clone XB | GU001720 | 98 | Sediment of gas plant | Shewanellaceae | HM193059 |
| BaSML19 | 17 | N | 0.7 | 2 | Shewanella frigidimarina NCIMB400 | Y13699 | 95 | S. frigidimarina | Shewanellaceae | HM193060 |
| BaSML20 | 17 | N | 12.8 | 4 | Shewanella sp. Lac1 | GQ327990 | 99 | Intestines of gastropoda | Shewanellaceae | HM193062 |
| Bacteroidetes | ||||||||||
| BaSML21 | 3 | P | 0.4 | 1 | Uncultured bacterium clone C6S16_ML_382 | FJ352992 | 95 | Lake water | Cryomorphaceae | HM193079 |
| BaSML22 | 3 | P | 2.8 | 2 | Uncultured Bacteroidetes bacterium clone PI_4z12e | AY580718 | 97 | Coastal water | Cryomorphaceae | HM193080 |
| BaSML23 | 4 | N | 1.3 | 1 | Uncultured bacterium clone S23_1172 | EF573073 | 98 | Marine water | Flavobacteriaceae | HM193057 |
| BaSML24 | 17 | N | 3.4 | 1 | Uncultured Flavobacterium isolate, DGGE gel band | EU878141 | 100 | Baltic Sea mesocosms | Flavobacteriaceae | HM193063 |
| BaSML25 | 11 | P | 0.4 | 1 | Uncultured Haliscomenobacter sp. clone XZXXH27 | EU703445 | 100 | Lake water | Saprospiraceae | HM193068 |
| Cyanobacteria | ||||||||||
| BaSML26 | 12 | N | 9.8 | 2 | Anabaena sp. BIR361 | EF568895 | 99 | Baltic Sea | Halospirulina | HM193054 |
| BaSML27 | 14, 17, 18 | N/P | 13.8 | 4 | Uncultured cyanobacterium clone B1-06-24L | FM212461 | 99 | Macrophyte associated | Halospirulina | HM193070 |
| BaSML28 | 17 | P | 3.8 | 1 | Anabaena sp. BIR358 | EF568894 | 99 | Baltic Sea | Halospirulina | HM193065 |
| BaSML29 | 17 | P | 7.4 | 1 | Uncultured Coelosphaerium sp. clone SC1-1 | EF638722 | 97 | Coastal water | Halospirulina | HM193066 |
| BaSML30 | 9, 10 | P | 6.0 | 2 | Nodularia spumigena strain NSLA01 | AF268009 | 99 | Nodularia spumigena | Halospirulina | HM193074 |
| BaSML31 | 17 | P | 1.9 | 1 | Uncultured organism clone Lc2yS22_ML_277 | FJ355041 | 99 | Lake water | Oscillatoria | HM193067 |
| BaSML32 | 13 | P | 1.6 | 2 | Uncultured eukaryote chloroplast gene | AB469950 | 99 | Cow manure compost | Oscillatoria | HM193069 |
| Other: BaSML33 | 2 | N | 2.4 | 1 | Uncultured bacterium clone mek61a11 | AY537289 | 94 | Unclassified | HM193055 | |
a
OTUs are grouped according to their phylogeny. The relative abundances of the OTUs are shown in Fig. S1 in the supplemental material.
b
See Table S1 in the supplemental material.
c
N, nonattached fraction; P, particle-attached fraction; N/P, OTUs found in both size fractions.
d
According to alignment to the NCBI database. DGGE, denaturing gradient gel electrophoresis.
e
According to alignment to the ARB database.