Fig. 5.

Ectopic expression of the active kinase domain in strains that lack either CfaD or AprA. The cfaD- and aprA-null strains were transformed with pifkA-14. Cell proliferation was followed in the presence (+) and absence (−) of coumermycin. Three independent experiments were performed. The error bars indicate SEM. The difference seen at the 144-h time point between cfaD-null cells and cfaD-null cells expressing Adm was significant (P < 0.05; Student's t test). In panel B, differences between transformed (both with and without coumermycin) and untransformed aprA-null cells at the 48-h through the 120-h time points were significant (P < 0.05).