Fig. 6.

Western analysis of extracellular CfaD and AprA during development in the Ax4 and BS167 strains. Cells growing in the presence of bacteria were harvested and plated for development in a manner that allows easy recovery of secreted proteins. Samples were collected at the indicated times (in hours poststarvation). After concentration, the samples were subjected to Western analysis. (A) The 58.5- and 27-kDa forms of CfaD detected using anti-CfaD antiserum. (B) The 60-kDa band of AprA detected using anti-AprA antiserum.