Fig. 4.

Hypoxic filamentation by C. albicans mutants. Single colonies of strains were grown on YPS agar at 25°C under an atmosphere of 0.2% O₂ and 6% CO₂; colonies were photographed after 3 days of growth. (A) Control strain CAF2-1, sch9 mutant CCS3, and sch9 rim15 double mutant AF1002 were compared. (B and C) Strains were grown on YPS medium without additions or in YPS medium containing 2 nM rapamycin or 1 mM caffeine. Strains included control strain CAF2-1, rim15 mutant CCS6, sch9 mutant CCS4, tpk1 mutant HPY300U, tpk2 mutant HPY400U, cph1 mutant JLC19, czf1 mutant CKY230, and ace2 mutant MK106.