Fig. 4.

Wsp1 is required for proper chitin distribution. (A) The nuclear morphology is altered as the result of the WSP1 gene disruption. Nuclear staining with DAPI was apparent in the wsp1 and wild-type strains, but the edges of the nuclei in the wsp1 mutant were rugged, in contrast to the wild-type strains, in which nuclei appeared as solid individual masses stained by DAPI. (B) The wild-type strain, and wsp1 WSP1 and WSP1-GBD strains showed an even distribution of CFW-stained material around the cell periphery, indicating normal chitin localization, in contrast to the wsp1 mutant, which showed aggregated chitin staining inside the cells. Cells were stained as described previously (28) and observed under a Zeiss Axio Imager 2 microscope.