Fig. 5.

Wsp1 is required for maintenance of proper actin dynamics. Rhodamine-conjugated phalloidin staining showed that actin cables and filaments were present in the wild-type, wsp1 WSP1 complemented, and WSP1-GBD domain-specific allele strains but disappeared from the wsp1 mutant. The cortical distribution of actin patches and filaments (indicated by arrows) are seen in the wild-type, wsp1 WSP1-complemented, and WSP1-GBD domain-specific allele strains. The wsp1 mutant showed faint background staining instead. The method of staining was as described previously (28).