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. 2011 Apr;10(4):565–577. doi: 10.1128/EC.00305-10

Fig. 5.

Fig. 5.

TEC1 downregulation by CLA is Ras1p dependent. (A) Quantitative Northern blot analysis was used to examine the kinetics of expression of TEC1 in SC5314 cells grown in Spider medium at 37°C in the absence or presence of 100 μM CLA. TEC1 transcript levels were quantified and normalized to those for the ACT1 loading control. Ratios were obtained by normalizing TEC1 transcript levels to those for untreated cells obtained at the 30-min time point, which were set as 100%. (B) TEC1 expression levels in the parental, ras1/ras1 (CDH107), tpk1/tpk1 (IIHB6), tpk2/tpk2 (TPO7.4), and efg1/efg1 (HLC52) strains grown as described for panel A for 90 min. TEC1 transcript levels were quantified and normalized to those for the ACT1 loading control. (C) Data presented are the relative TEC1 expression levels obtained by normalizing TEC1 transcript levels to those for parental untreated cells, which were set as 100%. Data are means and standard deviations of results from two independent hybridizations performed on duplicate biological samples. A single hybridization was performed using RNA isolated from the CAI4 and efg1/efg1 strains.