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The itr1a itr3c mutant had a lower inositol uptake rate than the wild-type H99. Inositol uptake assays were performed for C. neoformans strains (H99, CUX46, and CUX57) following the protocol described in Materials and Methods with several different concentrations of radioactively labeled inositol (0, 0.22 μM, 0.44 μM, 0.88 μM, and 3.68 μM). Representative results from three repeats were presented.
