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ROS production in strains. Cells were obtained from overnight-grown cultures, washed twice with PBS, and incubated with DCFDA, which specifically reacts with ROS in viable cells. We show that ROS in GOA31 (goa1Δ/goa1Δ) was increased but not in the WT or GOA32 (GOA1/goa1Δ) strain (**, P < 0.001).
