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. 2001 Aug 15;15(16):2134–2145. doi: 10.1101/gad.894801

Figure 3.

Figure 3

Extended occupancy of the hsp70 heat shock element (HSE) requires the HSF1 loop. (A) DNase I footprinting by chimeric heat shock transcription factor (HSF) proteins to the hsp70 HSE. HSF2Helix1 (mHSF2 in which aa 18–43 were substituted with aa 26– 51 from mHSF1) and HSF2Loop1 (mHSF2 aa 73–91 were substituted with aa 81–99 from mHSF1) were purified and used for DNase I footprint experiments, as described in Figure 2. The positions of the HSEs are shown by arrows. (B) Sequence of the hsp70 HSE with the five inverted HSE repeats are indicated with arrows. The boundaries of protection by HSF1 and HSF2 and the chimeras are indicated by the brackets.