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. 2001 Aug 15;15(16):2134–2145. doi: 10.1101/gad.894801

Figure 6.

Figure 6

The HSF1 loop confers heat shock inducible multimerization in vitro. (A) Temperature-dependent trimerization of HSF1. Purified HSF1 (4 μM) was incubated at 15°C, 32°C, or 42°C for 30 min and then subjected to EGS cross-linking (EGS concentration indicated by the wedge 0, 0.1, 0.2, 0.5, 1.0, 2.5 mM from left to right) and detection by Western blotting using a HSF1-specific antibody. The predicted monomer, dimer, trimer, and putative hexamers are indicated by the ovals. (B) The HSF1 loop confers heat inducible trimerization to HSF2. Purified HSF2 and HSF2loop1 proteins, incubated at 15°C, 32°C, or 42°C, were subjected to EGS cross-linking (0, 0.5, 2.5 mM left to right) and analyzed as in A.