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The HSF1 loop confers heat-inducible activation of Hsp gene expression in hsf1−/− mouse embryonic fibroblast (MEF) cells. Immunoblot analyses for levels of HSP70, HSP27, HSC70, and actin were performed on extracts prepared from control (C) or heat-shocked (HS; 42°C for 1 h, then at 37°C for 3 h ) hsf1−/− MEF cells transfected with pcDNA3.1 (V) or vector containing the indicated mouse HSF isoform. Extracts from isogenic wild-type (WT) MEFs are shown for comparison.
