Figure 8.

Expression of HSF2Loop1 in hsf1^−/− cells prevents heat-induced apoptosis. hsf1^−/− mouse embryonic fibroblasts (MEFs) were transfected with pcDNA3 alone (Vector) or harboring the indicated HSF sequence. Cells were exposed for 1 h to 44°C or kept at 37°C (control) and then cultured for another 24 h at 37°C prior to detection of apoptosis by flow cytometry of annexin V-FITC and propidium iodide (PI)-stained cells. The percentage of cells for each population of viable, apoptotic or apoptotic and necrotic, falling within each quadrant is indicated. Viable cells sort to the lower left quadrant.