Fig. 2.

Expression of the DNM1 and OLA1genes is largely unaffected by oxidative stress and by SKN7. RNA isolated from yeast strains JF1565 (SKN7 YAP1), JF1904 (skn7Δ), JF2081 (yap1Δ), and JF2312 (skn7Δ yap1Δ) was analyzed by Northern blot analysis for DNM1, OLA1, TRX2, and PGK1 gene expression. All strains were treated with 0.5 mM t-BOOH for 0, 10, 20, 30, or 40 min. The probes were as follows: DNM1 (+929/+1348), OLA1 (+878/+1205), and TRX2 (−160/+324). Values were normalized to PGK1 expression. Induction, shown below each panel, was calculated using the normalized value for the untreated (0 min) lane in the denominator.