Fig. 5.

Overexpression of YAP1 but not yap1 C303 suppresses oxidative stress sensitivity of skn7 I429A/V429A (A) and skn7 T437A (B) mutants. Oxidative stress assays of the yeast strain JF1904 containing plasmids carrying SKN7 (pXH1853), skn7 I428A/V429A (pXH1928), or skn7 T437A (pXH1854) with or without YAP1⁺ plasmids (pSEY18-R25, 2μ [high copy, hc], or pSM58, CEN [low copy, lc]) or yap1 mutant plasmids (pJAW1, pJAW76-2, pJAW6, p952, p976, or p951). Tenfold serial dilutions were spotted on YPD (untreated) or YPD plus 0.75 or 1 mM t-BOOH (treated) plates starting at 10⁶cells/ml. The plates were incubated at 30°C for 2 to 3 days.