Fig. 6.

Yap1p coprecipitation with Skn7p depends on residues within the n- and c-CRDs of Yap1p. Shown are the results of an in vitro coprecipitation assay in which bacterially expressed Skn7p-His6 (pSD2015) or His6 alone (pSD2012) was mixed with yeast protein extracts prepared from a yap1Δ skn7Δ strain (JF2312) carrying plasmids expressing various YAP1 alleles, including YAP1 (pSM58), yap1 C303A (p976), yap1 Δ220-243 (p951), yap1 CSE598A (pSMS38), and yap1 C629A (pSL-C629A). α-Yap1 antibody (a gift from S. Moye-Rowley) was used to detect Yap1p in the precipitate (left) and to examine Yap1 protein levels in the extracts (right).