Table 3.

Coimmunoprecipitation of Yap1p with Skn7p

Parameter	SKN7+a	skn7Δ	T455A	T437A	T449A	I428A/V429A	SKN7+yap1Δ
Avg Yap1/Skn7b	3.08	NDd	1.39	0.59*	0.58*	0.50*	ND
SD	1.34	–	0.18	0.2	0.06	0.04	–
% Recoveryc	100	–	45.1	19.2	18.8	16	–

^aProtein extracts were prepared from the skn7Δ strain, JF1904, carrying the SKN7-MYC plasmids pXH1853 (SKN7⁺), pXH1855 (skn7 T455A), pXH1854 (skn7 T437A), pXH1939 (skn7 T449A), or pXH1941 (skn7 I428A/V429A), as well as the YAP1 expression plasmid pSEY18-R2.5.

^bAverage Yap1/Skn7 values represent the average intensity of the Yap1p band from the immunoprecipitate relative to the intensity of the Skn7p band from the beads in each reaction mixture in three experiments. Asterisks indicate that the ratio for the mutant is significantly different (P<0.05) from the wild-type ratio. The skn7 T455A mutant, which is not oxidative stress sensitive, is included as a control.

^cThe percent Yap1p recovered is the relative amount of Yap1 protein in the immunoprecipitate of skn7 mutant extracts compared to that in SKN7 wild-type extracts.

^dND, not detectable.