Table 1.
Strains and plasmids used in this study
| Strain or plasmid | Genotype and/or relevant characteristicsa | Source or reference |
|---|---|---|
| Bacterial strains | ||
| HB101 | Nonpathogenic E. coli; normal food source for C. elegans | 11 |
| 14028 | S. Typhimurium wild-type | ATCCb |
| ΔsopB | ΔsopB Ω Kmr; Salmonella outer protein; homologue to ipgD of Shigella; regulated by SPI-1 | 58 |
| ΔsopD | ΔsopD Ω Kmr; secreted protein in the Sop family; transferred to eukaryotic cells; regulated by SPI-1 | 58 |
| ΔsopE2 | ΔsopE2 Ω Kmr; type III secreted protein effector; regulated by SPI-1 | 58 |
| ΔsipB | ΔsipB Ω Kmr; cell invasion protein; regulated by SPI-1 | 58 |
| ΔinvA | ΔinvA Ω Kmr; invasion protein; regulated by SPI-1 | 58 |
| ΔssaE | ΔssaE Ω Kmr; secretion system effector; regulated by SPI-2 | 58 |
| ΔsseB | ΔsseB Ω Kmr; secretion system effector; regulated by SPI-2 | 58 |
| ΔsseJ | ΔsseJ Ω Kmr; Salmonella translocated effector; regulated by SPI-2 | 58 |
| ΔadrA | ΔadrA Ω Kmr; putative diguanylate cyclase/phosphodiesterase domain 1 | 58 |
| ΔluxS | ΔluxS Ω Kmr; quorum-sensing protein, produces autoinducer signaling molecules | 58 |
| ΔphoP | ΔphoP Ω Kmr; response regulator in two-component regulatory system | 58 |
| ΔssrA | ΔssrA Ω Kmr; two-component regulatory system | 58 |
| 14028-GFP | 14028 wild type containing pCM18 | This study |
| ΔsopB-GFP | ΔsopB containing pCM18 | This study |
| 14028/SopB | 14028 wild type containing pSB2908 | This study |
| ΔsipB/SopB | ΔsipB containing pSB2908 | This study |
| ΔsopB/SopB | ΔsopB containing pSB2908 | This study |
| Candida strains | ||
| DAY185 | ura3Δ::λimm434/ura3Δ::λimm434 his1::hisG/HIS1::his1::hisG arg4::hisG/URA3::ARG4::arg4::hisG | 13 |
| Δtup1 | ura3Δ::λimm434/ura3Δ::λimm434 his1::hisG/tup1::hisG-URA3-hisG | 10 |
| Δsuv3 | ura3Δ::λimm434/ura3Δ::λimm434 suv3::Tn7-URA3/suv3::Tn7-UAU1 his1::hisG/his1::hisG arg4::hisG/arg4::hisG | 55 |
| SC5314 | Clinical isolate | 19 |
| Δcph1/Δefg1 | ura3Δ::λimm434/ura3Δ::λimm434 cph1Δ::hisG/cph1Δ::hisG efg1Δ::hisG/efg1Δ::hisG-URA3-hisG | 34 |
| SSY50-B | ura3Δ::λimm434/tet-NRG1/URA3 | 59 |
| Plasmids | ||
| pCM18 | Emr; pTRKL2-PCP25-RBSII-gfpmut3; broad host | 21 |
| pSB2908 | Amr; pBAD24 Plac::FLAG-tagged SopB+; arabinose inducible | 50 |
a
Amr, Emr, and Kmr represent ampicillin, erythromycin, and kanamycin resistance, respectively.
b
ATCC, American Type Culture Collection.