Table 2.
Oligonucleotides used for qRT-PCR
| Organism and gene | Orientationa | Sequence (5′-3′) |
|---|---|---|
| Salmonella | ||
| sopB | F | CTTATACAACGGAATGCAGATTCTC |
| R | AGTTATAGAGGTTATGCAGCGAGTG | |
| sipB | F | ATTACTGCTTGGCAAGTTAATGACC |
| R | TTTGATACTGGCTTCATAGAGATCC | |
| 16S rRNA | F | TGTAGCGGTGAAATGCGTAG |
| R | CAAGGGCACAACCTCCAAG | |
| Candida | ||
| TEC1 | F | TGGTGCTTATTCACGTGTCC |
| R | TTCTGAATTTCCCGGTTTTG | |
| HWP1 | F | CTCCAGCTGGCTCAAGTGGT |
| R | TGGCAGATGGTTGCATGAGT | |
| ALS3 | F | ACTTCCACAGCTGCTTCCAC |
| R | TGCAGATGGAGCATTACCAC | |
| CDC42 | F | AGGGTGAAAAATTGGCTAAGGA |
| R | TGCAGCTACTATAGCCTCGTCA | |
| 18S rRNA | F | GTGCCAGCAGCCGCGGTA |
| R | TGGACCGGCCAGCCAAGC |
a
F, forward primer; R, reverse primer.