Fig. 4.

Calcineurin and Crz1 control cation homeostasis in C. dubliniensis. (A) Cells were grown overnight in YPD at 30°C, 5-fold serially diluted, and spotted onto YPD medium containing CaCl₂, MnSO₄, or NaCl at the concentration indicated. The plates were incubated at 30°C for 48 h. (B) Quantitative real-time PCR was used to assay expression of genes involved in cation homeostasis (PMC1 and PMR1) or calcium channel (CCH1 and MID1) in the wild-type and mutant strains. The fold changes in transcription of each gene were normalized to the endogenous control ACT1. The error bars represent means ± SDs from a triplicate experiment. One representative figure of three independent experiments is shown. Asterisks indicate P < 0.001 compared with the wild type.