Fig. 1.

Gram-negative bacteria isolated from contaminated fuel samples. Partial 16S rRNA gene sequences (550 bp) were aligned, and the evolutionary history was inferred using the neighbor-joining method with a bootstrap value of 1,000 and the Jukes-Cantor algorithm. Sequences with a “JW” prefix represent fuel contaminants; the most closely related sequences were included as references. To simplify the tree, multiple isolates of the same genus/family were compressed at certain nodes; however, numbers of isolates in each group are shown. The tree was rooted by the Gram-positive species Bacillus cereus (EU048539).