Fig. 2.

Inhibition of P. aeruginosa choline catabolism by propargylcholine and its metabolites. (A) The percent growth of P. aeruginosa PA14 in the absence or presence of 500 μM PropCho, calculated from the OD₆₀₀ after 24 h of growth in MOPS minimal medium with a 20 mM concentration of the listed carbon source. Growth in the absence of PropCho was set at 100%. (B) Growth of P. aeruginosa wild type (wt), ΔbetBA, and ΔgbcA in the absence and presence of PC and PMG, as measured by absorbance at 600 nm. (C) ¹³C NMR results of ethanolic extracts from choline-grown cells exposed to [1,2-¹³C]PropCho. Peaks were validated by comparison to ¹³C NMR of chemically synthesized PropBet and PropMG. Error bars represent standard deviations of biological triplicates. All data are representative of at least three independent experiments.