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(A) Transcription of galP1 and galP2 promoters in the presence of GalR or wild-type HUα and HUα mutants, S17P, K18A, or T19D. Concentration of GalR was 80 nM; concentrations of HU were 40 nM, 80 nM, and 140 nM, as shown at the top. The 80-bp RNA1 served as an internal control. Details are in the text. (B) Graphical representation of the repression data for galP1 promoter. Transcription is expressed as the percentage of that which was obtained in the absence of GalR.
