Fig. 1.

Effect of the supernatants (Sn) of different strains of S. salivarius and S. vestibularis on TNF-α-induced NF-κB activation in HT-29/kB-luc-E. Luciferase activity was measured after addition of Sn to HT-29/luc-E reporter cells in the presence of TNF-α (10 ng/ml). The control assay corresponds to luciferase activity obtained with HT-29/kB-luc-E reporter cells in the presence of M17 bacterial growth medium with adjusted pH and TNF-α (10 ng/ml). Results are expressed as relative percentages of NF-κB activation compared to the control assay (100%). Results are represented by means ± standard deviations of triplicate measurements from one representative experiment out of a minimum of three independent experiments performed. Data were analyzed by Student's t test (P < 0.05 compared to control for all tested supernatants). Results from S. salivarius (white bars) and S. vestibularis (gray bars) are classified by increasing inhibition strength.