Fig. 2.
Determination of molecular mass and nature of the active component secreted by S. salivarius. (A) Fractions were obtained after filtrations of S. salivarius CIP102503T Sn (gray bars) and JIM8772 Sn (white bars) after CDM growth through selective membranes. Retained fractions containing metabolites of >10 kDa and >3 kDa and filtered fraction containing metabolites of <3 kDa were tested for inhibition of NF-κB transcription activity in HT-29/kB-luc-E cells. (B) S. salivarius JIM8772 <3-kDa Sn was treated by exposure to high temperature (100°C for 10 min), to heat shock (100°C for 10 min before freezing in liquid nitrogen), or to trypsin hydrolysis (200 U/ml). Results are from one representative out of at least three independent experiments. P values were <0.05 (*), <0.025 (**), or <0.01 (***) compared to control for the two tested strains. Bars represent standard deviations of the means.