Fig. 5.
The production of volatile and dissolved Te species from intracellular Te(0) by R. mucilaginosa strain 13B. The amounts of Te (in nmol) found in the precipitated (solid), dissolved (liquid), and volatile (traps) fractions after the indicated incubation times are noted. Values in italics are the relative amounts of DMTe (as the integrated DMTe count [×106]) in the headspace sample, determined by SPME-GC-MS analysis. Values in parentheses are the amounts of precipitated Te, calculated by adding all measurements for each replicate coming from this precipitate in the following experiments. The mass balance observed was 98% + 3%, indicating that all pools of Te were recovered. In sealed vials, the volatile Te could not determined at the nanomolar level, and these vials were thus not included in the calculation. The difference in the total amended tellurite is due to the difference in volume between the aerated (10 ml) and sealed (2 ml) culture volumes.