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. 2001 Apr 1;29(7):1616–1622. doi: 10.1093/nar/29.7.1616

Figure 1.

Figure 1

Overview of the bidirectional link between ESTs and cDNA-AFLP expression profiles established by GenEST. (A) From EST to cDNA-AFLP expression profiles. The predicted TDF of EST GE54 with primer extensions E+TC/T+AG and a size of 138 nt was readily identified on a cDNA-AFLP gel. (B) From cDNA-AFLP to EST. A band on the gel was amplified with primer extensions E+AA/T+TA with a size of 251 nt. These identifiers were used to search the virtual TDF list generated by GenEST. The corresponding EST was identified. M, molecular ladder. D, S, H, U and P represent the five different developmental stages of the potato cyst nematode: D, unhatched J2 in diapause; S, unhatched J2 after diapause, rehydrated for 2 days in water; H, freshly hatched J2 in PRD; U, developing nematodes (J1) in gravid females 2 months post-inoculation; P, developing nematodes (J2) in gravid females 3 months post-inoculation.