Fig. 3.

PsoR binds plant molecules. Soluble His₆-PsoR can be detected only in the presence of macerated plant material (see Materials and Methods for details). Affinity chromatography (A, C, and E) and Western blot analysis of each elution peak (B, D and F) using samples prepared under native conditions for E. coli containing pQEPsoR and pREP4 grown in LB supplemented with macerated cucumber leaves (A and B), macerated rice leaves (C and D), and macerated wheat leaves (E and F) were performed. Western blot analysis was carried out with anti-His₆ monoclonal antibody. FT, flowthrough. The 10, 20, and 50% refer to the percentages of buffer B used for elution, 50% buffer B corresponds to 135 mM imidazole.