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Quantitative serial PMCA of unbound HY TME prions. Results for triplicate 10-fold serial dilutions of HY TME agent-infected brain homogenate using two different PMCA protocols are shown. Error bars show ±1 standard error of the mean. y axes show the oral (p.o.), extranasal (e.n.), and intracerebral (i.c.) infectious titers of the brain homogenate dilutions (7). Values below 0 indicate less than 1 LD50.
