Table 1.
Parameters used to generate soil-bound prions
| Soil or minerala | Adsorption incubation time (h) | Soil concnb (mg/ml) | Brain homogenate (%) | Amt imaged for Fig. 1 (mg) | PMCA spike (1:100) (mg) |
|---|---|---|---|---|---|
| Rinda SCL soil | 24 | 5 | 0.5 | 0.5 | 0.1 |
| Bentonite clay | 24 | 5 | 0.5 | 0.5 | 0.1 |
| SiO2 powder | 24 | 50 | 0.25 | 5 | 2 |
| SiO2-HA | 168 | 10 | 0.5 | 1 | 1 |
| Fine quartz sand | 168 | 50 | 0.5 | 10 | 10 |
a
SCL, silty clay loam; SiO2, silicon dioxide; HA, humic acid.
b
The total adsorption solution volume was 1 ml (SCL soil, bentonite clay, SiO2, and SiO2-HA) or 200 μl (sand).