Fig. 1.

Two-dimensional polyacrylamide gel electrophoresis analyses of extracellular protein products of wild-type Edwardsiella ictaluri (WT) and T3SS regulatory gene mutants cultured in MM19 at pH 5.5. The T3SS translocon proteins EseB, EseC, and EseD and the T6SS protein EvpC are indicated by arrows. Circles indicate areas on the gel where protein spots occur in the WT ECP but are absent in the mutant ECP. Gel experiments were repeated three times, and representative gels are shown.