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Replication of Edwardsiella ictaluri strains carrying mutations in T3SS regulatory gene esrA (A), esrB (B), or esrC (C) in HKDM. Strains carrying complementation plasmids are labeled with pesrA, pesrB, or pesrC. Bars indicate the mean fold replication (± standard error of the mean [SEM]) at 5 and 10 h postinfection for triplicate assays. Asterisks indicate a significant difference from the WT fold increase at the same time point (P ≤ 0.05).
