Table 1.
Bacterial strains and plasmids used in this study
| Bacterial strain or plasmid | Description | Reference or source |
|---|---|---|
| Edwardsiella ictaluri | ||
| 93-146 | Wild-type E. ictaluri isolated from a moribund channel catfish from a natural outbreak at a commercial facility in 1993 | LSU aquatic animal diagnostic laboratory |
| 93-146 ΔesrA::km | 93-146 with nucleotides 112-2610 of esrA deleted in frame and a Tn5 kanamycin resistance cassette inserted; Kmr | This work |
| 93-146 ΔesrB::km | 93-146 with nucleotides 58-564 of esrB deleted in frame and a Tn5 kanamycin resistance cassette inserted; Kmr | This work |
| 93-146 ΔesrC | 93-146 with nucleotides 301-633 of esrC deleted in frame | This work |
| 93-146 ΔesrA::km/pesrA | 93-146 ΔesrA::km carrying pesrA; Kmr Apr | This work |
| 93-146 ΔesrB::km/pesrB | 93-146 ΔesrB::km carrying pesrB; Kmr Apr | This work |
| 93-146 ΔesrC/pesrC | 93-146 ΔesrC carrying pesrC; Apr | This work |
| WT/pesrA | 93-146 carrying pesrA; Apr | This work |
| WT/pesrB | 93-146 carrying pesrB; Apr | This work |
| WT/pesrC | 93-146 carrying pesrC; Apr | This work |
| EseB-Flag | 93-146 carrying pBBR1-EseB::Flag; Apr | This work |
| EseG-Flag | 93-146 carrying pBBR1-EseG::Flag; Apr | This work |
| EseH-Flag | 93-146 carrying pBBR1-EseH::Flag; Apr | This work |
| EseI-Flag | 93-146 carrying pBBR1-EseI::Flag; Apr | This work |
| EsrC-Flag | 93-146 carrying pBBR1-EsrC::Flag; Apr | This work |
| Escherichia coli | ||
| XL1 Blue MRF′ | (mcrA)183 (mcrCB-hsdSMR-mrr)173endA1 supE44 thi-1 recA1 gyrA96 relA1 lac [F′ proAB lacIqZM15 Tn5(Km)] | Stratagene, La Jolla, CA |
| CC118λ pir | Δ(ara-leu) araD ΔlacX74 galE galK phoA20 thi-1 rpsE rpoB argE(Am) recA1 λpir lysogen | 25 |
| SM10λ pir | thi1 thr1 leuB supE44 tonA21 lacY1 recA-::RP4-2-Tc::Mu Kmr λ::pir | 46 |
| Plasmids | ||
| pBluescript SK− | Cloning vector; Apr | Stratagene, La Jolla, CA |
| pGP704 | R6K ori mob; Apr; suicide vector used for allelic exchange | 35 |
| pRE107 | R6K ori mobsacB1; Apr; suicide vector used for allelic exchange | 16 |
| pGP::ΔesrA::km | pGP704 carrying ΔesrA::km | This work |
| pGP::ΔesrB::km | pGP704 carrying ΔesrB::km | This work |
| pRE::ΔesrC | pRE107 carrying ΔesrC | This work |
| pBBR1-MCS4 | Broad-host-range expression vector; Apr | 27 |
| pesrA | pBBR1-MCS4 carrying esrA | This work |
| pesrB | pBBR1-MCS4 carrying esrB | This work |
| pesrC | pBBR1-MCS4 carrying esrC | This work |
| pMRFlag | pBluescript SK-km::Flag with an SphI site engineered between km and the Flag epitope | This work |
| pBBR1-EseB::Flag | pBBR1-MCS4 carrying the escC/eseB ORFs fused to the Flag epitope | This work |
| pBBR1-EseG::Flag | pBBR1-MCS4 carrying the escB/eseG operon fused to the Flag epitope | This work |
| pBBR1-EseH::Flag | pBBR1-MCS4 carrying the eseH gene fused to the Flag epitope | This work |
| pBBR1-EseI::Flag | pBBR1-MCS4 carrying the escD/eseI operon fused to the Flag epitope | This work |
| pBBR1-EsrC::Flag | pBBR1-MCS4 carrying the esrC gene fused to the Flag epitope | This work |