Table 2.

Primers used for quantitative PCR in this study

Target	Primer	Primer sequence	Putative T3SS operon gene(s)a	Putative protein function(s)	GenBank accession no.
escB	5′	5′-CTTTACCTTGCGATTTGCCTGCGT-3′	escB, eseG	Chaperone and secreted effector	YP_002932393 and YP_002932394
	3′	5′-AACAGGCACTCCGCCATATGAAAC-3′
escC	5′	5′-AATGCAAGACCTACAGCAGCGTCA-3′	escC, escA, eseBCDE	Chaperone and translocon	YP_002932396 to YP_002932401
	3′	5′-GCGTTGCGATCTCTTGCTGTAACG-3′
eseH	5′	5′-AAGAGGCTGGATGTCTCTGGTACT-3′	eseH	Secreted effector	AF244083
	3′	5′-GGTAGCCTTGGCATAGGAGTGTTA-3′
eseI	5′	5′-GCAACCCTGCTTAGCAAGTGGAAA-3′	escD, eseI	Chaperone and secreted effector	AAF85960
	3′	5′-TCAAAGCCTTCTGGGCCTAATGGA-3′
esrA	5′	5′-AGAGCGAGCATCTGAACAGCATCA-3′	esrA	Two-component sensor kinase	YP_002932413
	3′	5′-AGTAAGTCATGCTGCTCCTGCGTA-3′
esrB	5′	5′-CAATGCAGCATGCATCACTGGGAA-3′	esrB	Two-component response regulator	YP_002932414
	3′	5′-TCAGCGATATCCCGGTTGCGATTA-3′
esrC	5′	5′-AAAGTTTGGGATGGCGCCGAA-3′	esaGHIKWKL	AraC-type regulator; apparatus	YP_002932388
	3′	5′-GAGAAATGGGCGGCGTTACAGAAT-3′
16S	5′	5′-AACGCGAAGAACCTTACCTGGTCT-3′
	3′	5′-GCTCGTTGCGGGAATTAAACCCAA-3′

^aTranscriptional linkages of underlined genes were confirmed by RT-PCR.