Table 3.
Primers used for fusion of the Flag epitope to T3SS proteins in this study
| Gene | Primer | Sequencea |
|---|---|---|
| km | 5′ | 5′-ATATATGAGCTCGAAGCCCTGCAAAGTAAA-3′ |
| 3′ | 5′-ATATATTCTAGACTATTTATCGTCGTCATCTTTGTAGTCGAAGAACTCGTCAAGAAGG-3′ | |
| kmFLAG | 5′ | 5′-ATATATGCATGCGAAGAACTCGTCAAG AAGGCG-3′ |
| 3′ | 5′-ATATATGCATGCTTCTTCGACTACAAAGATGACGAC-3′ | |
| eseB | 5′ | 5′-ATATATGAGCTCGCCCGCGTAAACAATC GACA-3′ |
| 3′ | 5′-ATATATTCTAGACTATTTATCGTCGTCATCTTTGTAGTCGCGGATATTCTGGGCAATGGA-3′ | |
| eseG | 5′ | 5′-ATATATAGATCTATCGGGCGCTGGATAA GATG-3′ |
| 3′ | 5′-ATATATGCATGCGGCAAAGCTGTGGCGTCG-3′ | |
| eseH | 5′ | 5′-ATATATGAGCTCAGCCATTCACGACACT GCAT-3′ |
| 3′ | 5′-ATATATGCATGCCAAGGAGTGATATACA GGGG-3′ | |
| eseI | 5′ | 5′-ATATATGAGCTCTGGCTCCCTAATCCTG TCTT-3′ |
| 3′ | 5′-ATATATGCATGCGGCTGGGATGAAGA CTCGC-3′ | |
| esrC | 5′ | 5′-ATATATGAGCTCCCAACCCTGACGCATC TTGAA-3′ |
| 3′ | 5′-ATATATGCATGCGCGGTGGTGATGACTGGC-3′ |
a
Underlined sequences are linkers incorporated into the primers used for cloning. Bold regions are the Flag-encoding DNA sequence. For eseB, eseG, and eseI, the cognate chaperone genes, escC, escB, and escD, respectively, were included in the amplicon.