Table 4.

Primers used for PCR in the mutagenesis of Edwardsiella ictaluri type III secretion system regulatory genes esrA, esrB, and esrC

Primer	Sequencea
esrA-P1	5′-TATATAGGTACCAACCCTACCCATATTGCC-3′
esrA-P2	5′-TATATATCTAGAGCTGCCACTGATTCGGAG-3′
esrA-P3	5′-TATATAGAATTCGTTCAGCAGCAGCGTCAC-3′
esrA-P4	5′-TATATAGAATTCGGCCTGACGTTGGTACAT-3′
esrB-P1	5′-ATATATGGTACCCGCGGGACATTATCAGGA-3′
esrB-P2	5′-ATATATTCTAGATGCGAGAAAAGCGCGATC-3′
esrB-P3	5′-TATATAGAATTCATTACGGATGCCATCGGC-3′
esrB-P4	5′-TATATAGAATTCAACCTGATGCGCAAGCTG-3′
esrC-P1	5′-TATATAGGTACCCGTCTGCAACGATACGCT-3′
esrC-P2	5′-TATATATCTAGACCATTGTTGATGAGGGCC-3′
esrC-P3	5′-TATATAGAATTCCAGCCTGAGCATGGTTTC-3′
esrC-P4	5′-TATATAGAATTCCACTTCAGTCAGTCGCCA-3′

^aUnderlined sequences indicate linkers incorporated into the primers used for cloning.