Table 5.
Primers used for the construction of complementation plasmids for ΔesrA::km, ΔesrB::km, and ΔesrC in this study
| Gene | Primer | Sequencea |
|---|---|---|
| esrA | 5′ | 5′-ATTTAATTTCTAGAATGCAGGTGATGCCG GAAA-3′ |
| 3′ | 5′-TTTAATTCTCGAGGCTGGAGGTTTAATCCGCCT-3′ | |
| esrB | 5′ | 5′-ATTTAATTTCTAGACGATGCATTCCACAA ATCCA-3′ |
| 3′ | 5′-ATTTAATTCTCGAGATACGCTAAAGGGGTT GGCC-3′ | |
| esrC | 5′ | 5′-TTTAATTTCTAGAATCGACTGCCTCAAT GACGC-3′ |
| 3′ | 5′-TTTAATTCTCGAGACCGTGACCATGTTTAGGCG-3′ |
Underlined sequences are linkers incorporated into the primers used for cloning.