Table 7.

Effects of T3SS regulatory gene mutations on expression of T3SS genes in Edwardsiella ictaluri cultured in MMP at pH 5.5

Mutant genotype	Fold change for target transcripta:
	escB	escC	eseH	eseI	esrA	esrB	esrC
ΔesrA::km	−6.3 A**	−4.8 A**	−3.9 A**	−1.6 A		+1.6 A	−6.0 A**
ΔesrB::km	−155.5 B**	−49.2 B**	−22.0 B**	−2.2 A	−2.3 A		−85.9 B**
ΔesrC	−11.9 A**	−2.1 A	−18.6 B**	−1.6 A	+2.3 B	+2.3 A
ΔesrC ΔesrB::km	−156.7 B**	−52.3 B**	−28.7 B**	−2.4 A	−2.5 A

^aValues are the fold increase or decrease in transcription of the T3SS genes relative to expression in WT E. ictaluri. Values within columns with the same letters are not significantly different (P > 0.01). Asterisks indicate a significant difference from WT: *, P ≤ 0.01; **, P ≤ 0.001.