Table 3.
Composition of media used to cultivate Clostridium spp.
| Component | Component concn (g/liter) in mediuma: |
|||||
|---|---|---|---|---|---|---|
| Consolidated medium of this study | Wiesenborn et al. (69) | Ounine et al. (54) | Lin and Blaschek (43) | Woolley and Morris (70) | Long et al. (45) | |
| KH2PO4 | 3 | 0.75 | 0.5 | 0.75 | 3 | |
| K2HPO4·3H2O | 0.5 | |||||
| K2HPO4 | 0.75 | 0.75 | ||||
| MgSO4·7H2O | 0.03 | 0.2 | 0.03 | 0.2 | ||
| MgSO4 | 0.4 | 0.02 | ||||
| MnSO4 | 0.01 | 0.01 | 0.01 | |||
| MnCl2·4H2O | 0.01 | 0.01 | 0.01 | |||
| FeSO4·7H2O | 0.01 | 0.01 | 0.01 | |||
| FeSO4·2H2O | 0.01 | 0.01 | 0.01 | |||
| NaCl | 0.01 | 1.0 | 0.01 | 1 | ||
| CH3COONH4 | 2.2 | |||||
| (NH4)2HPO4 | 6 | |||||
| NH4Cl | 3 | 3 | ||||
| (NH4)2SO4 | 0.5 | 2.0 | 2 | 0.5 | ||
| CaCl2 | 0.011 | 0.011 | ||||
| CaCO3 | 20 | |||||
| CuSO4·5H2O | 0.032 | 0.032 | ||||
| CoCl2·6H2O | 0.032 | 0.032 | ||||
| ZnSO4 | 0.044 | 0.044 | 0.05 | |||
| (NH4)6Mo7O24·4H2O | 0.022 | 0.022 | ||||
| Biotin | 0.001 | 1.10−5 | 0.001 | tr | ||
| p-Aminobenzoic acid | 0.002 | 0.001 | 0.002 | 0.001 | ||
| Thiamine HCl | 0.002 | 0.002 | 0.001 | |||
| Cysteine | 0.05 | 0.05 | 0.5 | |||
| Asparagine | 0.05 | 2.0 | 2 | |||
| Yeast extract | 1 | 5.0 | 10 | |||
| EDTA | 0.1 mM | |||||
| Tryptophan | 0.05 | |||||
| Histidine | 0.05 | |||||
| Glutamine | 0.05 | |||||
| Riboflavin | 0.001 | |||||
| Vitamin B12 | 0.001 | |||||
| Carbon source | d-Glucose/d-xylose | d-Glucose | Several | Corn starch | d-Glucose | d-Glucose |
a.
The first or leftmost column shows the composition of the consolidated medium utilized for the cultivation of Clostridium spp. used in this study. The medium was formulated after evaluation of meta-analysis data from cultures of C. acetobutylicum ATCC 824 exposed to butanol (Tables 1 and 2) and through comparisons to media described in the references shown. Concentrations are expressed in grams/liter except for EDTA, which is expressed in millimolar.