Table 4.
Composition of the validated medium formulated in this study to cultivate C. beijerinkii SA-1a
| Component of validated medium | Final concn |
|---|---|
| Medium components | |
| KH2PO4 | 3 g/liter |
| NH4Cl | 3 g/liter |
| (NH4)SO4 | 0.5 g/liter |
| NaCl | 0.01 g/liter |
| EDTA | 0.1 mM |
| Yeast extract | 2 g/liter |
| Tween 80 | 1 ml/liter |
| Trace componentsb | |
| MgSO4·7H2O | 0.1 g/liter |
| CaCl2 | 22 mg/liter |
| MnCl2·4H2O | 20 mg/liter |
| FeSO4·2H2O | 20 mg/liter |
| CuSO4·5H2O | 6.4 mg/liter |
| CoCl2·6H2O | 6.4 mg/liter |
| ZnSO4 | 8.8 mg/liter |
| (NH4)6Mo7O24·4H2O | 4.4 mg/liter |
| Biotin | 1 mg/liter |
| p-Aminobenzoic acid | 2 mg/liter |
| Thiamine HCl | 2 mg/liter |
| Cysteine | 0.1 g/liter |
| Asparagine | 0.1 g/liter |
| Tryptophan | 0.1 g/liter |
| Histidine | 0.1 g/liter |
| Glutamine | 0.1 g/liter |
| Riboflavin | 0.001 g/liter |
| Vitamin B12 | 0.001 g/liter |
a
Validated medium composition was obtained by applying the pulse-and-shift optimization technique (Fig. 3). The carbon source(s) is not included in the formula.
b
Trace components were prepared by filtration and aseptically added to the medium.