Table 7.
Results for C. beijerinckii SA-1/ATCC 35702 cultivated in a two-stage, one-feed-stream chemostat culture containing d-glucose and d-xylose in the validated mediuma
| Reactor | Overall D (h−1) | pH | Mean concn (g/liter) ± SD of the following fermentation product: |
Proportion (%) of sugar that was consumed |
Butanol yield (mol/mol of substrate) | No. of vegetative cells | No. of spores | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Acetate | Butyrate | Acetone | Butanol | d-Glucose | d-Xylose | ||||||
| First reactor | 0.102 | 6.5 | 3.3 ± 0.6 | 6.3 ± 0.5 | 0.2 ± 0.1 | 0.5 ± 0.3 | 69.2 | 31.0 | |||
| Second reactor | 0.04 | 6.5 | 1.6 ± 0.8 | 6.4 ± 3.0 | 0.2 ± 0.1 | 0.4 ± 0.2 | 93.3 | 59.1 | 0.018 | 7.3 E7 | 2.3 E8 |
| 0.05 | 6.5 | 1.2 ± 1.0 | 4.0 ± 0.8 | 0.5 ± 0.2 | 1.8 ± 0.8 | 96.9 | 67.6 | 0.078 | 1.4 E8 | 1.5 E8 | |
| 0.06 | 6.5 | 2.0 ± 1.0 | 1.6 ± 1.0 | 5.3 ± 0.6 | 7.2 ± 0.2 | 83.2 | 52.9 | 0.380 | 1.8 E8 | 1.3 E8 | |
| 0.04 | Free | 2.7 ± 0.2 | 3.2 ± 1.2 | 1.1 ± 0.1 | 3.7 ± 0.4 | 95.7 | 47.5 | 0.183 | |||
| 0.05 | Free | 2.0 ± 0.4 | 3.5 ± 0.2 | 0.5 ± 0.1 | 2.0 ± 0.1 | 96.6 | 32.7 | 0.105 | |||
| 0.06 | Free | 2.0 ± 0.1 | 3.2 ± 0.9 | 0.4 ± 0.1 | 2.1 ± 0.5 | 93.5 | 57.6 | 0.100 | |||
a
C. beijerinckii SA-1/ATCC 35702 was cultivated in a two-stage, one-feed-stream chemostat culture containing d-glucose (40 g/liter) and d-xylose (20 g/liter) in the validated medium described in Table 4 at 37°C and pH 6.5. The dilution rate (D) and pH were varied in the second reactor.