Table 2.
Intraspecies differentiation of C. gasigenes, TC1, C. estertheticum, and C. algidixylanolyticum using RFLP, SR-PCR, and a combination of the two methodsa
| Microorganism | RFLP |
SR-PCR |
RFLP and SR-PCR |
||||||
|---|---|---|---|---|---|---|---|---|---|
| No. of typeable isolates | SV (%) | C (%) (no. of clusters) | No. of typeable isolates | SV (%) | C (%) (no. of clusters) | No. of typeable isolates | SV (%) | C (%) (no. of clusters) | |
| C. gasigenes | 208 | 53.3 | 75 (7) | 300 | 54.6 | 80 (5) | 208 | 54.2 | 76.5 (7) |
| TC1 | 52 | 50.9 | 82.9 (5) | 51 | 61.3 | 92.1 (3) | 51 | 63.1 | 85.9 (2) |
| C. estertheticum | 18 | 60.8 | 80 (3) | 19 | 75.7 | 86.3 (2) | 18 | 72.3 | 80.7 (2) |
| C. algidixylanolyticum | 2 | 33.3 | 33.3 (1) | 13 | 72.3 | 89.9 (2) | 2 | 71.4 | 71.4 (1) |
a
SV, similarity value (percent); C, percent cluster cutoff.