Colony and mycelium traits of the M. anisopliae wild type (MA35505) and transformant (MA05-169) on PDA. (A) Incubation on PDA at 33°C and 34°C for 25 days; at 34°C, growth of both strains was restricted, particularly M. anisopliae BCRC35505. (B) Conidia (c) of M. anisopliae BCRC35505 on PDA at 34°C for 7 days germinated, but most germ tubes (g) were abnormal, swollen (s), or burst (b) (arrowheads); in contrast, the conidia of M. anisopliae MA05-169 mostly exhibited normal growth, though occasionally the tip dissolved (arrowheads).