Table 1.
Transcript copy numbers and gene copy numbers of melanin biosynthesis genes, PKS, SCD, and THR, in A. alternata BCRC30501, M. anisopliae BCRC35505 (wild type) and M. anisopliae MA05-169 (transformant) estimated by absolute Q-PCR and Southern blotting, respectively
| Strain | Transcript copy no.a |
Gene copy no. |
||||
|---|---|---|---|---|---|---|
| PKS | SCD | THR | PKSb | SCDc | THRc | |
| A. alternata BCRC30501 | 22.2 × 104 | 1.9 × 104 | 4.5 × 104 | 1 | 1 | 1 |
| M. anisopliae BCRC35505 | NA | NA | NA | 0 | 0 | 0 |
| M. anisopliae MA05-169 | 4.0 × 104 | 7.9 × 104 | 1.2 × 106 | 2 | 2 | 2 |
a
NA, not applicable.
b
gDNAs digested with PstI.
c
gDNAs digested with HindIII.