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HPLC graphs of steroid extracts from β-sitosterol bioconversions after 3 days of incubation. (A) Profile of mutant strain RG32ΔfadD19 showing the accumulation of metabolites (degradation pathway intermediates) that, based on identical HPLC retention times, were identified as 4-sitostene-3-one (I) and 4-campestene-3-one (II). (B) Profile of parent strain RG32.
