Table 3.
Whole-cell bioconversion of sterols/steroids into ADD and 1,4-BNC by R. rhodochrous mutant strain RG32 after 72 h of incubationa
| Steroid substrate | ADD (molar %) | 1,4-BNC (%) |
|---|---|---|
| 4-Androstene-3,17-dione | 69 (±9) | NA |
| 23,24-Bisnorchol-5-en-22-oic acid-3β-ol | − | 60 (±21) |
| Campesterol | 1 (±0) | 49 (±9) |
| 5-Cholenic acid-3β-ol | 1 (±0) | 77 (±4) |
| 4-Cholestene-3-one | 4 (±1) | 79 (±2) |
| 1,4-Cholestadiene-3-one | 3 (±1) | 71 (±4) |
| Cholesterol | 3 (±1) | 73 (±12) |
| β-Sitosterol | 7 (±2) | 67 (±7) |
a
NA, not applicable; −, not detected. The data represent the molar % conversion as averages of triplicate experiments; standard deviations are shown in parentheses.