Table 4.
Whole-cell bioconversion of sterols by R. rhodochrous RG32 and mutants thereof into ADD and 1,4-BNC, measured after 72 h of incubationa
| Strain | % conversion of sterol: |
|||||
|---|---|---|---|---|---|---|
| Cholesterol |
β-Sitosterol |
Campesterol |
||||
| ADD | 1,4-BNC | ADD | 1,4-BNC | ADD | 1,4-BNC | |
| RG32 | 3 (±1) | 73 (±12) | 7 (±2) | 67 (±7) | 1 (±0) | 49 (±9) |
| RG32Δro04690DSM43269 | 4 (±1) | 74 (±8) | 7 (±4) | 54 (±9) | ND | ND |
| RG32Δro04690DSM43269 ΔfadE26 | 3 (±1) | 72 (±4) | 6 (4) | 61 (±9) | ND | ND |
| RG32Δro04690DSM43269 ΔfadE26 ΩfadE27 | 3 (±2) | 70 (±6) | 7 (±2) | 65 (±6) | ND | ND |
| RG32ΩfadD17 | 3 (±2) | 74 (±9) | 8 (±2) | 64 (±5) | ND | ND |
| RG32ΔfadD19 | 6 (±2) | 64 (±4) | − | − | − | − |
| RG32ΔfadD19+pCOMPfadD19DSM43269 | ND | ND | 6 (±1) | 60 (±4) | ND | ND |
| RG32ΔfadD19 ΩfadD17 | 6 (±2) | 69 (±1) | − | − | ND | ND |
a
ND, not determined; −, not detected. The data represent averages of duplicate experiments; standard deviations are shown in parentheses.