Fig. 7.

(A) Northern analysis of cel7a (cbh1) transcription. Strains were grown on Mandels Andreotti minimal medium with 1% (wt/vol) microcrystalline cellulose as carbon source for 72 h and 96 h in constant light (LL) (1,800 lx, 25 μmol photons m⁻² s⁻¹) or constant darkness (DD). Representative hybridizations with 18S rRNA are shown below the respective series. (B) Western blot analysis of CEL7A from culture filtrates of wild-type QM9414 and Δgrd1 strains during growth in liquid cultures on Mandels Andreotti minimal medium with 1% (wt/vol) microcrystalline cellulose as carbon source for 72 h and 96 h in constant light (1,800 lx, 25 μmol photons m⁻² s⁻¹) or constant darkness. Equal amounts of culture filtrate were used.