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. 2001 Sep 1;15(17):2282–2294. doi: 10.1101/gad.205501

Figure 3.

Figure 3

Gel mobility-shift assay for ADP · AlFx-dependent complex formation between the PspFΔHTH T86S GAFTGA mutant and ς54 detected by Coomassie staining. Reactions contained PspFΔHTH T86S (20 μM) and ς54 (2 μM). Arrow (a), trapped ς54–PspFΔHTH T86S complex; (b), PspFΔHTH T86S–ADP · AlFx complex.